Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary
Mouse Lipocalin-2/NGAL Quantikine ELISA Kit Summary
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货号：MLCN20
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL)
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse Lipocalin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
196
|
476
|
1534
|
211
|
477
|
1564
|
|
Standard Deviation
|
13.2
|
19.6
|
65.9
|
15.2
|
28.6
|
98.5
|
|
CV%
|
6.7
|
4.1
|
4.3
|
7.2
|
6
|
6.3
|
Recovery
The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Samples (n=4)
|
93
|
82-101
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.
