Human ST2/IL-33 R Quantikine ELISA Kit
Human ST2/IL-33 R Quantikine ELISA Kit
Human ST2/IL-33 R Quantikine ELISA Kit Summary
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货号：DST200
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ST2 as well as free ST2 and IL-33 complexed ST2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human ST2/IL-33 R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ST2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human ST2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ST2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ST2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
273
|
628
|
1027
|
262
|
642
|
1064
|
|
Standard Deviation
|
15.2
|
27.5
|
46.3
|
18.7
|
34.7
|
67.2
|
|
CV%
|
5.6
|
4.4
|
4.5
|
7.1
|
5.4
|
6.3
|
Recovery
The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
97
|
89-106
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
