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Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit

Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit

 详情说明


Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

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货号:RLB00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Specificity
Natural and recombinant rat IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.


Product Summary

The Quantikine Rat IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 beta in cell culture supernates, serum, and EDTA plasma. It contains recombinant rat IL-1 beta and antibodies raised against recombinant rat IL-1 beta. This immunoassay has been shown to quantitate the recombinant rat factor accurately.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 83.4 253 610 82.3 249 626
Standard Deviation 7.3 9.9 24 4.7 10.3 27
CV% 8.8 3.9 3.9 5.7 4.1 4.4

Recovery

The recovery of rat IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 100 89-110
EDTA Plasma (n=5) 98 89-111
Serum (n=5) 108 97-120

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
Rat IL-1 beta/IL-1F2 ELISA Linearity

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