Human MMP-9 Quantikine ELISA Kit
Human MMP-9 Quantikine ELISA Kit
Human MMP-9 Quantikine ELISA Kit Summary
Catalog # Availability Size
DMP900 1 Kit
SMP900 1 Pack (6 Plates)
PDMP900 1 Pack (50 Plates)
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL), Saliva (10 uL), Urine (100 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human 92 kDa Pro-MMP-9 and the 82 kDa active MMP-9
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human MMP-9 Immunoassay is a 3.5 hour solid phase ELISA designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form) in cell culture supernates, saliva, serum, plasma, and urine. It is calibrated with CHO cell-expressed recombinant human pro-MMP-9 and the antibodies were raised against the recombinant factor. Natural human MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human MMP-9.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.833
|
2.04
|
11
|
0.972
|
2.35
|
12.2
|
|
Standard Deviation
|
0.017
|
0.039
|
0.316
|
0.077
|
0.184
|
0.845
|
|
CV%
|
2
|
1.9
|
2.9
|
7.9
|
7.8
|
6.9
|
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