/

全国统一客服热线:
0755-28715175

产品中心
Product
4产品中心
Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit

Mouse IL-10 Quantikine ELISA Kit

 详情说明

Mouse IL-10 Quantikine ELISA Kit Summary

Catalog                Size / Price
M1000B              1 Kit (2 Plates) / Please Inquire
SM1000B            1 Pack (6 Plates) / Please Inquire

PM1000B            1 Pack (50 Plates) / Please Inquire

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
5.22 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-10 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision over two lots of kit components. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 76.3 123 815 71.4 115 809
Standard Deviation 2.56 2.85 17 5.39 7.64 55.5
CV% 3.4 2.3 2.1 7.5 6.6 6.9

Recovery

The recovery of IL-10 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 99 95-103
EDTA Plasma (n=4) 91 86-99
Heparin Plasma (n=4) 89 82-98
Serum (n=4) 89 85-96
深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

Copyright © 深圳9游总区生物科技有限公司版权所有

扫一扫,加关注

客户服务热线:
0755-33164177
13924667705
18665335907
0755-28715175


线