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脱氧核糖核酸酶II CAS : 9025-64-3
脱氧核糖核酸酶II CAS : 9025-64-3
脱氧核糖核酸酶II CAS : 9025-64-3

脱氧核糖核酸酶II CAS : 9025-64-3

 详情说明

脱氧核糖核酸酶II CAS : 9025-64-3

货号:zk4771

英文名: Deoxyribonuclease II
别名: Deoxyribonuclease II
级别Activity ≥800 U/mg dw
储存冷藏(2-8℃)
规格:80 KU

产品描述

概述

Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

技术规格

Source Porcine Spleen
Description A dialyzed, lyophilized powder.
Storage Store at 2-8°C
Activity ≥800 units per mg dry weight
Unit Definition One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.

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