Synonyms :

TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand, TL2

Description :

TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell's apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

Catalog #:

310-04

Source :

E.coli

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analysis.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Assay#1: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED ₅₀ for this effect is 1.0-3.0 ng/ml.
Assay#2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED ₅₀ for this effect is 0.8 - 2.0 ng/ml.

Selected References :

AA Sequence :

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

PubMed Link :

Country of Origin :

USA

Special Sizes: 
Many products can be ordered in custom sizes. Please  to contact with your request.

Usage: 
For Research Use Only. Not for use in diagnostic or therapeutic procedures.