Mouse Progranulin Quantikine ELISA Kit
Mouse Progranulin Quantikine ELISA Kit
Mouse Progranulin Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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MPGRN0
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Contact your distributor for availability.
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1 Kit /
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.6 - 20 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse Progranulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Mouse Progranulin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Progranulin in cell culture supernates, tissue culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Progranulin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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1.75
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2.94
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7.28
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1.69
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2.89
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7.53
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Standard Deviation
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0.05
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0.067
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0.16
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0.092
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0.137
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0.336
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CV%
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2.9
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2.3
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2.2
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5.4
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4.7
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4.5
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Recovery
The recovery of mouse Progranulin spiked to three levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Culture Media (n=4)
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98
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94-104
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Progranulin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
