Human Calbindin D Quantikine ELISA Kit
Human Calbindin D Quantikine ELISA Kit
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Human Calbindin D Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DCALD0
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Tissue Lysates (10 uL), Urine (25 uL)
Assay Range
0.1 - 5 ng/mL
Specificity
Natural and recombinant human Calbindin D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Calbindin D Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Calbindin D in tissue lysates and urine. It contains E. coli-expressed recombinant human Calbindin D and antibodies raised against the recombinant factor. Natural human Calbindin D showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural human Calbindin D.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Tissue Lysates, Urine
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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0.511
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1.65
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2.84
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0.548
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1.57
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2.96
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Standard Deviation
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0.026
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0.04
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0.072
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0.038
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0.075
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0.15
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CV%
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5.1
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2.4
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2.5
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6.9
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4.8
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5.1
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Recovery
The recovery of human Calbindin D spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Urine (n=4)
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93
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89-98
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human Calbindin D were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
