Mouse IL-6 R alpha Quantikine ELISA Kit
Mouse IL-6 R alpha Quantikine ELISA Kit
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Mouse IL-6 R alpha Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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MR600
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 12 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-6 R alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-6 R alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-6 R alpha levels in cell culture supernates, serum, and heparin plasma. It contains NS0-expressed recombinant mouse IL-6 R alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-6 R alpha accurately. Results obtained using natural mouse IL-6 R alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6 R alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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0.456
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1.07
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2.94
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0.474
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1.08
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2.82
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Standard Deviation
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0.036
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0.039
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0.148
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0.06
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0.08
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0.2
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CV%
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7.9
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3.6
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5
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12.7
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7.4
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7.1
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Recovery
The recovery of mouse IL-6 R alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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98
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90-110
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Heparin Plasma (n=4)
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99
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92-106
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Serum (n=4)
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102
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93-110
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 R alpha were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
