Human Uteroglobin Quantikine ELISA Kit
Human Uteroglobin Quantikine ELISA Kit
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Human Uteroglobin Quantikine ELISA Kit Summary
货号：DUGB00 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Uteroglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Saliva, Urine, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
5.1
|
15.4
|
31.3
|
5.2
|
15.4
|
29.9
|
|
Standard Deviation
|
0.29
|
0.34
|
1.04
|
0.31
|
0.72
|
2.25
|
|
CV%
|
5.7
|
2.2
|
3.3
|
6
|
4.7
|
7.5
|
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
5.2
|
15.8
|
31.7
|
5
|
14.7
|
28.8
|
|
Standard Deviation
|
0.21
|
0.49
|
1.16
|
0.28
|
0.92
|
2.09
|
|
CV%
|
4
|
3.1
|
3.7
|
5.6
|
6.3
|
7.3
|
Recovery
The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
99-109
|
|
EDTA Plasma (n=4)
|
95
|
85-107
|
|
Heparin Plasma (n=4)
|
95
|
86-109
|
|
Serum (n=4)
|
95
|
85-104
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
