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Mouse Chemerin Quantikine ELISA Kit
Mouse Chemerin Quantikine ELISA Kit
Mouse Chemerin Quantikine ELISA Kit

Mouse Chemerin Quantikine ELISA Kit

 详情说明

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Mouse Chemerin Quantikine ELISA Kit Summary

货号:MCHM00  规格:96T

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
3.47 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse Chemerin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natural mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 153 405 1124 168 385 1065
Standard Deviation 13 16.2 99 14.2 25.7 64.1
CV% 8.5 4 8.8 8.4 6.7 6

Recovery

The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 102 89-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse Chemerin in each matrix were diluted with Calibrator Diluent and assayed.
Mouse Chemerin ELISA Linearity

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