HIV-1 Gag p24 Quantikine ELISA Kit
HIV-1 Gag p24 Quantikine ELISA Kit
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HIV-1 Gag p24 Quantikine ELISA Kit Summary
货号：DHP240
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
HIV-1 Gag p24.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine HIV-1 Gag p24 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure HIV-1 Gag p24 in cell culture supernates, serum, and plasma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
45.7
|
135
|
280
|
43
|
132
|
285
|
|
Standard Deviation
|
1.41
|
4.86
|
8.1
|
1.93
|
4.61
|
11.2
|
|
CV%
|
3.1
|
3.6
|
2.9
|
4.5
|
3.5
|
3.9
|
Recovery
The recovery of HIV-1 Gag p24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
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Sample Type
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Average % Recovery
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Range %
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|
Cell Culture Media (n=4)
|
100
|
93-105
|
|
EDTA Plasma (n=4)
|
74
|
64-82
|
|
EDTA Plasma (ICD Treated) (n=4)
|
92
|
85-100
|
|
Heparin Plasma (n=4)
|
72
|
63-78
|
|
Heparin Plasma (ICD Treated) (n=4)
|
88
|
69-94
|
|
Serum (n=4)
|
79
|
73-85
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of HIV-1 Gag p24 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
