Rat CXCL2/CINC-3 Quantikine ELISA Kit
Rat CXCL2/CINC-3 Quantikine ELISA Kit
Rat CXCL2/CINC-3 Quantikine ELISA Kit Summary
货号：RCN300
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant rat CINC-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat CINC-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
50
|
46
|
50
|
|
Mean
|
111
|
345
|
1012
|
105
|
308
|
877
|
|
Standard Deviation
|
3.9
|
11.9
|
39.1
|
10.1
|
28.5
|
73.2
|
|
CV%
|
3.5
|
3.4
|
3.9
|
9.6
|
9.3
|
8.3
|
Recovery
The recovery of rat CINC-3 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
97
|
86-107
|
|
EDTA Plasma (n=6)
|
92
|
86-99
|
|
Heparin Plasma (n=6)
|
99
|
85-105
|
|
Serum (n=6)
|
97
|
83-106
|
Linearity
To assess the linearity of the assay, samples containing rat CINC-3 in each matrix were diluted with Calibrator Diluent and then assayed.
