Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit
Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit
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Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit Summary
货号：MAFP00 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse AFP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse alpha -Fetoprotein/AFP Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse AFP levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse AFP and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse AFP accurately. Results obtained using natural mouse AFP showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse AFP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
|
1
|
2
|
3
|
|
n
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20
|
20
|
20
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20
|
20
|
20
|
|
Mean
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0.404
|
1.12
|
3.3
|
0.457
|
1.2
|
3.38
|
|
Standard Deviation
|
0.013
|
0.026
|
0.091
|
0.031
|
0.066
|
0.158
|
|
CV%
|
3.2
|
2.3
|
2.8
|
6.8
|
5.5
|
4.7
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Recovery
The recovery of mouse AFP spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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98
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93-105
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse AFP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
