Human DPPIV/CD26 Quantikine ELISA Kit
Human DPPIV/CD26 Quantikine ELISA Kit
Human DPPIV/CD26 Quantikine ELISA Kit Summary
货号：DC260B
深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human DPPIV.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
2.16
|
8.15
|
14.1
|
2.22
|
8.25
|
14.6
|
|
Standard Deviation
|
0.105
|
0.28
|
0.916
|
0.14
|
0.695
|
1.18
|
|
CV%
|
4.9
|
3.4
|
6.5
|
6.3
|
8.4
|
8.1
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
2.32
|
8.78
|
15.8
|
2.43
|
8.53
|
15.9
|
|
Standard Deviation
|
0.142
|
0.354
|
1.15
|
0.207
|
0.777
|
1.3
|
|
CV%
|
6.1
|
4
|
7.3
|
8.5
|
9.1
|
8.2
|
Recovery
The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
101
|
87-113
|
|
Urine (n=4)
|
101
|
88-113
|
Linearity
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
