Human GM-CSF Quantikine HS ELISA Kit
Human GM-CSF Quantikine HS ELISA Kit
Human GM-CSF Quantikine HS ELISA Kit Summary
货号：HSGM0
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (200 uL)
Assay Range
1.0 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma), 0.5 - 32 pg/mL (Urine)
Specificity
Natural and recombinant human GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
2.69
|
7.05
|
24.8
|
3.77
|
14.3
|
27.4
|
|
Standard Deviation
|
0.14
|
0.33
|
0.73
|
0.37
|
1.17
|
1.85
|
|
CV%
|
5.2
|
4.7
|
2.9
|
9.8
|
8.2
|
6.8
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
18
|
18
|
18
|
|
Mean
|
3.68
|
22
|
44.6
|
2.8
|
16.2
|
33.2
|
|
Standard Deviation
|
0.35
|
1.07
|
1.76
|
0.66
|
2.47
|
4.6
|
|
CV%
|
9.5
|
4.9
|
4
|
23.5
|
15.3
|
13.9
|
Recovery
The recovery of GM-CSF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
EDTA Plasma (n=4)
|
114
|
88-140
|
|
Heparin Plasma (n=4)
|
109
|
91-122
|
|
Serum (n=4)
|
109
|
94-128
|
|
Urine (n=5)
|
102
|
95-116
|
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of GM-CSF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
