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Human BMP-4 Quantikine ELISA Kit
Human BMP-4 Quantikine ELISA Kit
Human BMP-4 Quantikine ELISA Kit

Human BMP-4 Quantikine ELISA Kit

 详情说明

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Human BMP-4 Quantikine ELISA Kit Summary

货号:DBP400

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
Sensitivity
3.68 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts)
Specificity
Natural and recombinant human BMP-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure BMP-4 levels in bone tissue extracts and cell culture supernates. It contains NS0-expressed recombinant human BMP-4 and antibodies raised against the recombinant factor.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Bone Extracts

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 301 719 1351 289 734 1429
Standard Deviation 15.9 22.5 60.1 16.9 42.7 75.4
CV% 5.3 3.1 4.4 5.8 5.8 5.3

Recovery

The recovery of recombinant human BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=2) 96 93-103
2 M Urea (n=2) 97 91-105
Cell Culture Media (n=8) 100 91-109

Linearity

The following samples were spiked with rhBMP-4 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess linearity of the assay.
Human BMP-4 ELISA Linearity

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