Human Total MMP-8 Quantikine ELISA Kit
Human Total MMP-8 Quantikine ELISA Kit
Human Total MMP-8 Quantikine ELISA Kit Summary
货号：DMP800B
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (50 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva)
Specificity
Natural and recombinant human MMP-8.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Total MMP-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total MMP-8 (pro- and active MMP-8) in cell culture supernates, tissue lysates, serum, plasma, platelet-poor plasma, and saliva. It contains NS0-expressed recombinant human MMP-8, and antibodies raised against the recombinant protein. Natural human MMP-8 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MMP-8.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
0.764
|
2.38
|
4.55
|
0.836
|
2.41
|
5.07
|
|
Standard Deviation
|
0.049
|
0.07
|
0.299
|
0.055
|
0.171
|
0.27
|
|
CV%
|
6.4
|
2.9
|
6.6
|
6.6
|
7.1
|
5.3
|
Recovery
The recovery of human Total MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
98
|
93-103
|
|
EDTA Plasma (n=4)
|
92
|
86-99
|
|
Extraction Buffer (1X) (n=2)
|
84
|
83-85
|
|
Heparin Plasma (n=4)
|
92
|
86-99
|
|
Platelet-poor EDTA Plasma (n=4)
|
85
|
79-91
|
|
Platelet-poor Heparin Plasma (n=4)
|
84
|
78-89
|
|
Saliva (n=4)
|
96
|
86-111
|
|
Serum (n=4)
|
101
|
93-109
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total MMP-8 in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
