Human IL-4 Quantikine HS ELISA Kit
Human IL-4 Quantikine HS ELISA Kit
Human IL-4 Quantikine HS ELISA Kit Summary
Catalog Size / Price
HS400 1 Kit / Please Inquire
SS400 1 Pack (6 Plates) / Please Inquire
PHS400 1 Pack (50 Plates) / Please Inquire
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL)
Assay Range
0.3 - 16 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant human IL-4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine HS Human IL-4 Immunoassay is a 6.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
1.75
|
5.56
|
10.7
|
0.59
|
3.32
|
10.7
|
|
Standard Deviation
|
0.05
|
0.28
|
0.29
|
0.06
|
0.19
|
0.79
|
|
CV%
|
2.9
|
5
|
2.7
|
10.2
|
5.7
|
7.4
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
0.68
|
3.79
|
12.3
|
0.61
|
3.56
|
11.1
|
|
Standard Deviation
|
0.05
|
0.3
|
0.94
|
0.06
|
0.29
|
0.9
|
|
CV%
|
7.4
|
7.9
|
7.6
|
9.8
|
8.1
|
8.1
|
Recovery
The recovery of IL-4 was determined by spiking to levels throughout the range of the assay in various matrices.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=9)
|
106
|
87-118
|
|
Serum (n=4)
|
102
|
95-108
|
