Human TIMP-2 Quantikine ELISA Kit
Human TIMP-2 Quantikine ELISA Kit
Human TIMP-2 Quantikine ELISA Kit Summary
货号：DTM200
规格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-2 levels in cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human TIMP-2, and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.23
|
3.45
|
6.09
|
1.26
|
3.45
|
6.38
|
|
Standard Deviation
|
0.054
|
0.116
|
0.181
|
0.086
|
0.197
|
0.467
|
|
CV%
|
4.4
|
3.4
|
3
|
6.8
|
5.7
|
7.3
|
Cell Culture Supernates, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.01
|
2.9
|
5.26
|
1.01
|
2.79
|
5.27
|
|
Standard Deviation
|
0.042
|
0.173
|
0.344
|
0.079
|
0.188
|
0.326
|
|
CV%
|
4.2
|
6
|
6.5
|
7.8
|
6.7
|
6.2
|
Recovery
The recovery of TIMP-2 spiked to three different levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
95
|
81-108
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
