Mouse/Rat IL-33 Quantikine ELISA Kit
Mouse/Rat IL-33 Quantikine ELISA Kit
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Mouse/Rat IL-33 Quantikine ELISA Kit Summary
货号:M3300
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IL-33
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natural IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
41
|
41
|
41
|
|
Mean
|
58.2
|
298
|
630
|
57.6
|
282
|
599
|
|
Standard Deviation
|
5.6
|
17.4
|
33.5
|
5.89
|
16.3
|
30
|
|
CV%
|
9.6
|
5.8
|
5.3
|
10.2
|
5.8
|
5
|
Recovery
The recovery of IL-33 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=4)
|
104
|
91-116
|
|
Mouse EDTA Plasma (n=4)
|
102
|
96-113
|
|
Mouse Heparin Plasma (n=4)
|
99
|
83-111
|
|
Mouse Serum (n=4)
|
90
|
81-103
|
|
Rat Cell Culture Supernates (n=4)
|
104
|
94-116
|
|
Rat EDTA Plasma (n=4)
|
93
|
83-100
|
|
Rat Heparin Plasma (n=4)
|
93
|
87-99
|
|
Rat Serum (n=4)
|
93
|
86-101
|
