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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：PLB00B
Cell Culture Supernates, Serum, Heparin Plasma
The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (50 uL), Heparin Plasma (50 uL)
Sensitivity
13.6 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant porcine IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
136
348
780
141
338
783
Standard Deviation
9.8
17
40.9
12.2
20.5
31.7
CV%
7.2
4.9
5.2
8.7
6.1
4
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
106
102-109
Heparin Plasma (n=4)
111
98-117
Serum (n=4)
111
102-117
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
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