Human Vitamin D BP Quantikine ELISA Kit
Human Vitamin D BP Quantikine ELISA Kit
Human Vitamin D BP Quantikine ELISA Kit Summary
货号：DVDBP0B
规格：96T
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Assay Range
6.3 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human Vitamin D BP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Vitamin D BP immunoassay is a 3.5 hour sandwich-type solid phase ELISA designed to measure human Vitamin D BP in cell culture supernates, serum, plasma, and urine. It contains HEK293-expressed recombinant human Vitamin D BP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Vitamin D BP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Vitamin D BP.
Read more about the Vitamin D BP specificity in this .
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
10.9
|
31.7
|
63.7
|
10.1
|
30.8
|
61.9
|
|
Standard Deviation
|
0.244
|
0.423
|
1.36
|
0.661
|
0.664
|
1.31
|
|
CV%
|
2.2
|
1.3
|
2.1
|
6.5
|
2.2
|
2.1
|
Recovery
The recovery of human Vitamin D BP spiked to three different levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
101
|
93-117
|
|
Urine (n=4)
|
89
|
78-96
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Vitamin D BP were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
