热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
货号：D5000B 规格：96T
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates, Urine
The recovery of human IL-5 spiked to different levels throughout the range of the assay in various matrices was evaluated.
Human IL-5 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
1.08 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
31.3
95.6
173
32.9
97.9
180
Standard Deviation
1.4
3.5
6.5
4
6.7
8.4
CV%
4.5
3.7
3.8
12.2
6.8
4.7
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
28.1
89.3
170
31.2
92.6
174
Standard Deviation
0.89
2
7.27
2.5
4.2
8.7
CV%
3.2
2.2
4.2
8
4.5
5
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
101
95-107
EDTA Plasma (n=4)
104
86-118
Heparin Plasma (n=4)
101
87-112
Serum (n=4)
104
88-116
Urine (n=4)
96
91-106
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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