热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
货号：MGD150 规格：96T
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
Mouse/Rat GDF-15 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
2.2 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural mouse and rat GDF-15, recombinant mouse mature GDF-15, precursor GDF-15, and GDF-15/BMP-2 heterodimer. It does not detect monomeric recombinant mouse GDF-15.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
25.6
60
190
26.7
63.1
196
Standard Deviation
0.69
1.42
5.49
1.62
2.84
10.7
CV%
2.7
2.4
2.9
6.1
4.5
5.5
Recovery
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Media (n=4)
102
91-110
Mouse EDTA Plasma (n=4)
95
81-109
Mouse Heparin Plasma (n=4)
95
87-104
Mouse Serum (n=4)
91
82-101
Mouse Urine (n=4)
97
88-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 in each matrix were diluted with Calibrator Diluent and assayed.
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