Human VEGF-C Quantikine ELISA Kit
Human VEGF-C Quantikine ELISA Kit
Human VEGF-C Quantikine ELISA Kit Summary
货号:DVEC00
规格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Assay Range
109.0 - 7,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma, Saliva)
Specificity
Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
572
|
1622
|
3243
|
520
|
1527
|
3069
|
|
Standard Deviation
|
39.3
|
54.4
|
136
|
50.1
|
102
|
200
|
|
CV%
|
6.9
|
3.4
|
4.2
|
9.6
|
6.7
|
6.5
|
Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
512
|
1543
|
3195
|
514
|
1540
|
3066
|
|
Standard Deviation
|
33.8
|
54.2
|
136
|
43.6
|
110
|
198
|
|
CV%
|
6.6
|
3.5
|
4.3
|
8.5
|
7.2
|
6.4
|
Recovery
The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
107
|
93-115
|
|
EDTA Plasma (n=4)
|
101
|
96-110
|
|
Heparin Plasma (n=4)
|
97
|
88-103
|
|
Serum (n=4)
|
99
|
89-106
|
