Human CCL20/MIP-3 alpha Quantikine ELISA Kit
Human CCL20/MIP-3 alpha Quantikine ELISA Kit
Human CCL20/MIP-3 alpha Quantikine ELISA Kit Summary
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货号：DM3A00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MIP-3 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
66.2
|
180
|
372
|
68.7
|
186
|
357
|
|
Standard Deviation
|
1.84
|
3.83
|
15.1
|
4.51
|
13
|
28.8
|
|
CV%
|
2.8
|
2.1
|
4.1
|
6.6
|
6.9
|
8.1
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
55.9
|
161
|
323
|
72.5
|
200
|
386
|
|
Standard Deviation
|
1.62
|
4.28
|
9.1
|
6.05
|
17.9
|
35.3
|
|
CV%
|
2.9
|
2.7
|
2.8
|
8.3
|
8.9
|
9.1
|
Recovery
The recovery of MIP-3 alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=8)
|
101
|
88-115
|
|
EDTA Plasma (n=5)
|
104
|
97-115
|
|
Heparin Plasma (n=5)
|
104
|
98-112
|
|
Serum (n=5)
|
103
|
94-112
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of MIP-3 alpha in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
