Human sTfR Quantikine IVD ELISA Kit
Human sTfR Quantikine IVD ELISA Kit
Human sTfR Quantikine IVD ELISA Kit Summary
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货号：DTFR1
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL)
Assay Range
3.0 - 80 nmol/L (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Measures human sTfR levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with other substances.
Product Summary
The Quantikine IVD Human sTfR Immunoassay is designed for the quantitative determination of soluble transferrin receptor (sTfR) concentrations in human serum and plasma as an aid in the diagnosis of Iron-Deficiency Anemia (IDA), especially for the differential diagnosis of IDA and Anemia of Chronic Disease (ACD). For In Vitro Diagnostic Use.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
60
|
60
|
60
|
|
Mean
|
6.9
|
18.2
|
40.2
|
10.9
|
25.7
|
67.2
|
|
Standard Deviation
|
0.3
|
1.3
|
2.5
|
0.7
|
1.4
|
3.8
|
|
CV%
|
4.3
|
7.1
|
6.2
|
6.4
|
5.4
|
5.7
|
Recovery
The recovery of sTfR spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Citrate Plasma (n=5)
|
112
|
105-121
|
|
EDTA Plasma (n=5)
|
103
|
97-112
|
|
Heparin Plasma (n=5)
|
106
|
101-112
|
|
Serum (n=5)
|
97
|
95-100
|
Linearity
To assess the linearity of the assay, five samples containing high concentrations of sTfR were diluted with Specimen Diluent and evaluated.
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