/

全国统一客服热线:
0755-28715175

产品中心
Product
4产品中心
Human Osteopontin (OPN) Quantikine ELISA Kit Summary
Human Osteopontin (OPN) Quantikine ELISA Kit Summary
Human Osteopontin (OPN) Quantikine ELISA Kit Summary

Human Osteopontin (OPN) Quantikine ELISA Kit Summary

 详情说明

Human Osteopontin (OPN) Quantikine ELISA Kit Summary

深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

货号:DOST00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
0.024 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human Osteopontin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 2.3 4.9 9.3 2.36 4.81 9.17
Standard Deviation 0.092 0.126 0.267 0.155 0.274 0.499
CV% 4 2.6 2.9 6.6 5.7 5.4

Recovery

The recovery of Osteopontin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 86-113
EDTA Plasma (n=4) 102 92-114
Heparin Plasma (n=4) 101 95-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Osteopontin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Osteopontin/OPN ELISA Linearity

Copyright © 深圳9游总区生物科技有限公司版权所有

扫一扫,加关注

客户服务热线:
0755-33164177
13924667705
18665335907
0755-28715175


线