Human CCL17/TARC Quantikine ELISA Kit
Human CCL17/TARC Quantikine ELISA Kit
Human CCL17/TARC Quantikine ELISA Kit Summary
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货号：DDN00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human TARC Immunoassay kit is a 3.5 hour solid phase ELISA designed to measure TARC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human TARC and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TARC. Results obtained using natural human TARC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TARC.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
87
|
600
|
1107
|
98
|
616
|
1178
|
|
Standard Deviation
|
6.2
|
13.7
|
29.9
|
8.9
|
50.7
|
88.8
|
|
CV%
|
7.1
|
2.3
|
2.7
|
9.1
|
8.2
|
7.5
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
88
|
504
|
1001
|
89
|
540
|
1033
|
|
Standard Deviation
|
4.1
|
21.7
|
27.2
|
8.3
|
38.9
|
71.7
|
|
CV%
|
4.7
|
4.3
|
2.7
|
9.3
|
7.2
|
6.9
|
Recovery
The recovery of TARC spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
95
|
88-104
|
|
EDTA Plasma (n=5)
|
102
|
86-111
|
|
Heparin Plasma (n=5)
|
98
|
86-107
|
|
Serum (n=5)
|
105
|
100-114
|
