Human TIMP-1 Quantikine ELISA Kit
Human TIMP-1 Quantikine ELISA Kit
Human TIMP-1 Quantikine ELISA Kit Summary
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货号：DTM100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TIMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human TIMP-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure TIMP-1 concentrations in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human TIMP-1 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human TIMP-1 accurately. Results obtained using natural TIMP-1 showed linear curves that were parallel to the standard curve obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural TIMP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.48
|
1.27
|
6.95
|
0.51
|
1.28
|
6.9
|
|
Standard Deviation
|
0.02
|
0.05
|
0.35
|
0.02
|
0.05
|
0.34
|
|
CV%
|
4.2
|
3.9
|
5
|
3.9
|
3.9
|
4.9
|
Recovery
The recovery of TIMP-1 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
102
|
94-113
|
|
EDTA Plasma (n=5)
|
99
|
93-108
|
|
Heparin Plasma (n=5)
|
99
|
89-108
|
|
Saliva (n=4)
|
105
|
89-121
|
|
Serum (n=5)
|
98
|
87-102
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TIMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
