Human EGF Quantikine ELISA Kit Summary
Human EGF Quantikine ELISA Kit Summary
Human EGF Quantikine ELISA Kit Summary
货号：DEG00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (10 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Platelet-poor Citrate Plasma (200 uL), Urine (10 uL)
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma, Urine)
Specificity
Natural and recombinant human EGF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
15
|
78.9
|
134
|
15
|
80
|
136
|
|
Standard Deviation
|
0.4
|
2.5
|
4
|
0.9
|
4.5
|
6.8
|
|
CV%
|
2.7
|
3.2
|
3
|
6
|
5.6
|
5
|
Serum, EDTA Plasma, Heparin Plasma, Platelet-poor Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
13.5
|
80
|
133
|
14.7
|
77
|
134
|
|
Standard Deviation
|
0.6
|
2
|
2.7
|
0.9
|
3.3
|
6.1
|
|
CV%
|
4.4
|
2.5
|
2
|
6.1
|
4.3
|
4.6
|
Recovery
The recovery of EGF spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
98
|
88-111
|
|
Citrate Plasma (n=10)
|
104
|
89-115
|
|
EDTA Plasma (n=10)
|
100
|
80-111
|
|
Heparin Plasma (n=10)
|
96
|
79-108
|
|
Serum (n=10)
|
97
|
80-106
|
|
Urine (n=1)
|
102
|
97-108
|
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