Mouse/Rat IGF-I Quantikine ELISA Kit
Mouse/Rat IGF-I Quantikine ELISA Kit
Mouse/Rat IGF-I Quantikine ELISA Kit Summary
货号：MG100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat IGF-I Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IGF-I in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse IGF-I and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse and rat IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat IGF-I.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
35
|
35
|
35
|
|
Mean
|
82
|
269
|
921
|
86
|
256
|
863
|
|
Standard Deviation
|
4.6
|
8.9
|
37.7
|
7.8
|
11.8
|
37.4
|
|
CV%
|
5.6
|
3.3
|
4.1
|
9.1
|
4.6
|
4.3
|
Recovery
The recovery of mouse/rat IGF-I spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=7)
|
98
|
89-111
|
|
Rat Cell Culture Supernates (n=6)
|
98
|
82-108
|
Linearity
To assess the linearity of the assay, samples containing mouse or rat IGF-I in each matrix were diluted with Calibrator Diluent and then assayed.
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