Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary
货号：DSA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Assay Range
156.0 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity
Natural and recombinant SDF-1 alpha
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
491
|
1700
|
3420
|
544
|
1600
|
3150
|
|
Standard Deviation
|
19
|
58
|
115
|
73
|
150
|
257
|
|
CV%
|
3.9
|
3.4
|
3.4
|
13.4
|
9.4
|
8.2
|
Recovery
The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
101
|
95-108
|
|
Platelet-poor EDTA Plasma (n=5)
|
97
|
90-107
|
|
Platelet-poor Heparin Plasma (n=5)
|
97
|
90-104
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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