Human IGF-I Quantikine ELISA Kit
Human IGF-I Quantikine ELISA Kit
Human IGF-I Quantikine ELISA Kit Summary
Catalog Size / Price
DG100 1 Kit / Please Inquire
SG100 1 Pack (6 Plates) / Please Inquire
PDG100 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
Assay Range
0.1 - 6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
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Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.49
|
1.243
|
2.373
|
0.382
|
0.967
|
1.941
|
|
Standard Deviation
|
0.017
|
0.054
|
0.103
|
0.031
|
0.08
|
0.145
|
|
CV%
|
3.5
|
4.3
|
4.3
|
8.1
|
8.3
|
7.5
|
Recovery
The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
99
|
97-102
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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