Human Total Adiponectin/Acrp30 Quantikine ELISA Kit
Human Total Adiponectin/Acrp30 Quantikine ELISA Kit
Human Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary
Catalog Size / Price
DRP300 1 Kit / Please Inquire
SRP300 1 Pack (6 Plates) / Please Inquire
PDRP300 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
3.9 - 250 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
19.8
|
69.9
|
143
|
20.5
|
74.4
|
157
|
|
Standard Deviation
|
0.5
|
2.4
|
6.76
|
1.4
|
4.3
|
10.8
|
|
CV%
|
2.5
|
3.4
|
4.7
|
6.8
|
5.8
|
6.9
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
12.5
|
45.3
|
91.5
|
12.7
|
47.1
|
100
|
|
Standard Deviation
|
0.41
|
1.27
|
4.56
|
1
|
2.8
|
6.7
|
|
CV%
|
3.2
|
2.8
|
5
|
7.9
|
5.9
|
6.7
|
Recovery
The recovery of Adiponectin spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
101
|
88-112
|
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