Human TNF-alpha Quantikine HS ELISA
Human TNF-alpha Quantikine HS ELISA
Human TNF-alpha Quantikine HS ELISA Summary
Catalog Size / Price
HSTA00E 1 Kit / Please Inquire
SSTA00E 1 Pack (6 Plates) / Please Inquire
PHSTA00E 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
0.2 - 10 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human TNF-alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® HS Human TNF-alpha Immunoassay is a 4.0 hour solid phase ELISA designed to measure human TNF-alpha in serum and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained with natural human TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
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1.07
|
3.3
|
6.71
|
1.09
|
3.26
|
6.51
|
|
Standard Deviation
|
0.024
|
0.066
|
0.129
|
0.073
|
0.22
|
0.406
|
|
CV%
|
2.2
|
2
|
1.9
|
6.7
|
6.7
|
6.2
|
Recovery
The recovery of human TNF-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
EDTA Plasma (n=4)
|
97
|
80-111
|
|
Heparin Plasma (n=4)
|
96
|
80-111
|
|
Serum (n=4)
|
94
|
83-103
|
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