Human CCL2/MCP-1 Quantikine ELISA Kit
Human CCL2/MCP-1 Quantikine ELISA Kit
Human CCL2/MCP-1 Quantikine ELISA Kit Summary
Catalog # Availability Size / Price
DCP00 1 Kit / Please Inquire
SCP00 1 Pack (6 Plates) / Please Inquire
PDCP00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
76.7
|
364
|
1121
|
74.2
|
352
|
1076
|
|
Standard Deviation
|
6
|
17.1
|
54.4
|
5
|
20.5
|
49.4
|
|
CV%
|
7.8
|
4.7
|
4.9
|
6.7
|
5.8
|
4.6
|
Cell Culture Supernates, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
78.2
|
360
|
1079
|
76
|
360
|
1086
|
|
Standard Deviation
|
3.3
|
17.7
|
63.5
|
4.5
|
17.2
|
48.5
|
|
CV%
|
4.2
|
4.9
|
5.9
|
5.9
|
4.8
|
4.5
|
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