Human IL-8/CXCL8 Quantikine ELISA Kit
Human IL-8/CXCL8 Quantikine ELISA Kit
Human IL-8/CXCL8 Quantikine ELISA Kit Summary
Catalog Size / Price
D8000C 1 Kit / Please Inquire
S8000C 1 Pack (6 Plates) / Please Inquire
PD8000C 1 Pack (50 Plates)
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-8
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural human IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) hree samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
168
|
526
|
1093
|
196
|
581
|
1160
|
|
Standard Deviation
|
9.4
|
28.6
|
70.7
|
14.5
|
56.6
|
70.2
|
|
CV%
|
5.6
|
5.4
|
6.5
|
7.4
|
9.7
|
6.1
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
115
|
386
|
802
|
132
|
410
|
817
|
|
Standard Deviation
|
5.3
|
17
|
37.9
|
10.7
|
28
|
42.4
|
|
CV%
|
4.6
|
4.4
|
4.7
|
8.1
|
6.8
|
5.2
|
Recovery
The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
98
|
85-114
|
|
Citrate Plasma (n=5)
|
105
|
95-114
|
|
EDTA Plasma (n=5)
|
103
|
97-111
|
|
Heparin Plasma (n=5)
|
102
|
92-107
|
|
Serum (n=5)
|
98
|
88-106
|
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-8 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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